#PYMOL TUTORIAL HIDE SHOW CHAIN HOW TO#

Ab-initio surface-based docking with HADDOCK.

Preparing PDB files of the ligands for docking.

Preparing PDB files of the receptor for docking.This tutorial consists of the following sections: Thus our structure suggests a transport pathway not through the central pore but through the identified channels in the individual subunits, which greatly advances our understanding of the multidrug export mechanism.” There seems to be remote conformational coupling between the channel access, exit, and the putative proton-translocation site, explaining how the proton motive force is used for drug export. … The three subunits of AcrB are locked in different conformations revealing distinct channels in each subunit. “The multidrug exporter AcrB is the inner membrane component of the AcrAB-TolC drug efflux system in Escherichia coli and is responsible for the resistance of this organism to a wide range of drugs. Sennhauser G, Amstutz P, Briand C, Storchenegger O, Grütter MG Using as example the multidrug exporter AcrB, described in the following publication:ĭrug export pathway of multidrug exporter AcrB revealed by DARPin inhibitors. Here is the final look of this jump ring necklace.In this tutorial we will use HADDOCK in its ab-initio mode to try to identify putative binding sites for small ligands on a protein receptor, Step 3: Add chain to the jump ring patterġst, slide a pink round jade bead to 4 eyepins respectively and hang them to the big jump rings on top Ģnd, make other 2 jump ring patterns with 12 big jump rings and 12 small jump rings as pictured ģrd, attach 2 round bead patterns to each jump ring pattern as pictured Ĥth, make other 4 round jade bead patterns and cut off 4 pieces of golden chain (2 short and 2 long), then attach the 4 chains to the jump ring patterns and link them with the jump ring pendant together~ġst, make other round jade bead patterns and cut off other 2 pieces of twisted chain Ģnd, attach one end of the chains to the jump ring pattern ģrd, attach a jump ring to the other end of one chain while attach a spring clasp to the other end of the other chain. Step 2: Add pink glass beads to the jump ring patternġst, add 2 small jump rings to the right jump ring on bottom, then attach other 4 small jump rings to them as pictured Ģnd, repeat above process in this step to add 6 small jump rings to other big jump rings on bottom ģrd, slide a pink drop glass bead to 3 headpins respectively and hang them to the small jump rings as pictured.

#PYMOL TUTORIAL HIDE SHOW CHAIN HOW TO#

Instructions on how to make beaded chain necklace: Step 1: Make a jump rings patternġst, combine 4 big jump rings (with same sizes) with 2 small jump rings and stay them as 2 parts Ģnd, add other 2 big jump rings together with the first 2 pater through other 4 small jump rings as pictured ģrd, add 2 big jump rings under the ready jump ring pattern through other 4 small jump rings Ĥth, add 2 big jump rings to both sides respectively of the jump ring pattern and fix them with 4 small jump rings for each side ĥth, add 4 big jump rings on bottom of the jump ring pattern and fix them with small jump rings Ħth, add 2 big jump rings on bottom of the jump ring pattern and fix them with 4 small jump rings as pictured. Supplies you’ll need in making this golden chain necklace: See this golden chain necklace, it is a 2-strand bead and chain necklace with jump rings and glass beads as pendant, how do you like it? Now I will show you the detail steps in making this chain bead necklace.

Pymol tutorial hide show chain